Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Medchemexpress LLC 2-Morpholinoethanesulphonic acid hemisodium | 117961-21-4 | 98.0% | 100 G
MES (2-Morpholinoethanesulphonic acid) hemisodium is an amphoteric ion buffer with a buffer capacity ranging from pH 5.5-7.0. As a Good's buffer, it is widely utilized in biochemistry and molecular biology experiments, including cell culture, enzyme activity determination, electrophoresis, and protein studies.
- It is a zwitterionic buffer and provides effective buffering capacity around pH 6.15.
- The pKa value changes with temperature, requiring consideration for its use at different temperatures.
- It shows good buffering properties in biological experiments.
- It exhibits poor affinity with metal ions, allowing its use in studies involving metal ions.
Bioworld EGTA 0.5M, pH 8.0, DNase and RNase Free, 50 mL
EGTA Buffer is of a 0.5M concentration and pH of 8.0 that is DNase and RNase free. EGTA Buffer is a metal chelating agent specific for calcium ions. EGTA Buffer is commonly used in tissue culture media and biological buffers.
Sigma Aldrich Fine Chemicals Biosciences MOPS solution BioPerforman1L
MOPS SOLUTIONon BioPerforman1L
Hampton Research 100% TACSIMATE PH 8.0 200ML
HR2-829/100% Tacsimate pH 8.0 - 200 ml. Please note items from this supplier are non-returnable.
Growcells PBS 20X 10L EA
Sterile 20X Phosphate-Buffered Saline solution concentrate prepared with 18.2 megOhm water, 0.2 micron sterile filtered and steam sterilized in final packaging. Recipe/L: NaCl 160g, KCl 4g, Na2HPO4 28.8g, KH2PO4 4.8g
Research Products International Corp PBS, 1X Solution, 1.0ml Pre-Filled Tubes, S, 1000 CS
PBS (Phosphate-Buffered Saline) is a balanced salt solution that helps maintain the pH and osmolarity of experimental systems, making it ideal for various biological and biochemical experiments.
1X Solution refers to a diluted concentration of a particular solution. In the case of PBS, a 1X solution means that it is prepared by diluting the concentrated stock solution to a specific ratio, usually 1 part stock solution to 9 parts water or another suitable solvent. 1.0ml Pre-Filled Tubes are small tubes that come pre-filled with a specified volume of liquid, in this case, 1.0 milliliters of PBS. These tubes are convenient and sterile, ensuring the integrity and purity of the solution. They are commonly used in laboratory settings to provide a controlled and standardized amount of PBS for various applications.
![Medchemexpress LLC (3-{[Tris(hydroxymethyl)methyl]amino}propane-1-sulfonic acid) | 29915-38-6 | 98.0% | 50 G](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000409966-logo.png-250.jpg){kind=logo}
Medchemexpress LLC (3-{[Tris(hydroxymethyl)methyl]amino}propane-1-sulfonic acid) | 29915-38-6 | 98.0% | 50 G
TAPS is an aminosulfonate-based lysozyme stabilizer and connexin channel inhibitor. It helps maintain the native structure of lysozyme in aqueous solution at pH 7.0 and protects it from heat-induced denaturation. TAPS is also considered a critical compound for investigating the structure and function of connexin channels.
- Maintains native structure of lysozyme in aqueous solution at pH 7.0
- Protects lysozyme from heat-induced denaturation
- Directly inhibits heteromeric connexin 32/connexin 26 channels (Cx32/Cx26)
- Reduces connexin channel-mediated solute exchange in a recombinant liposome system
- Critical compound for investigating connexin channel structure and function
Gold Biotechnology Inc HEPES Free Acid 2 x 1 kg
HEPES is a buffering agent used in biochemistry and molecular biology that was selected and described by Good et al It is a zwitterionic piperazinic buffer that is useful for a pH range of 6 8 8 2 HEPES lacks the ability to form a significant complex with most metal ions and is recommended for use as a non-coordinating buffer in solutions with metal ions
Quality Biological Inc Tris HCl (2 M) pH 8.8 500mL
Tris solutions are formulated into working concentrations for preparing the stacking and resolving portions of native or SDS-PAGE gels using discontinuous buffer systems according to Laemmli (1970) or Ornstein and Davis (1959). Tris HCl Buffer is compatible with many enzymes in molecular biology (e.g. DNA modifying enzymes). Use the Tris-HCl 0.5 M (pH 6.8) buffer for stacking gels. Use Tris-HCl 1.5 M (pH 8.8) buffer for resolving gels. Use Tris-HCl 1M pH 7.0-7.5 for DEAE Dextran-mediated transfection of non-adherent and adherent cells.Manufactured in an ISO 90012015 certified facility.
Sigma Aldrich Fine Chemicals Biosciences HEPES sodium salt solution1L
HEPES is a general-purpose zwitterionic buffer which does not bind magnesium calcium manganese(II) or copper(II) ions. HEPES has been classified as Good buffer by Dr. Norman Good and his colleagues in 1966. It is zwitterionic at biological pH and is most effective in the pH range of pH 6.8 to 8.2.